FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_POK5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_POK5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49177869
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2566700.016.62311794-95
CGCCGTA3183200.016.03172194-95
CCGTATC1990100.015.941998594-95
CATTAAA994350.015.76570494-95
TATACCG151300.014.3778795
TCATTAA1117250.014.11860694-95
CGTATCA1623100.013.864264594-95
TCGCCGT3899000.012.59020894-95
ATCATTA1236850.012.358726592-93
TAGAGCG137300.012.3153775
CCTAGAC136050.011.9048553
GTCTAGG135750.011.57951
TGGTCGC4582900.011.37097790-91
TATCATT1342050.011.2377692-93
CTAGACT141700.011.2290554
GTCGCCG4726950.011.14938792-93
GGTCGCC4539850.011.12496392-93
GTATAGG220350.011.0248541
GTATCAT1446550.011.01623294-95
TAGACTG137900.010.8151785
Produced by FastQC (version 0.11.5)