FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_POK180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_POK180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98697120
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTA4335200.022.77011994-95
TCATTAA3288650.022.74675894-95
TAGACGG96200.021.7241335
TATCATT5509900.021.37375894-95
GTACCGT154100.021.0206726
CATTAAA2343750.020.83632994-95
GTATCAT6739300.019.51925394-95
TATACCG247050.019.0910245
GTCTAGG232700.018.9966491
AGTACCG216900.018.0220895
CCTAGAC250200.017.5787733
GGACTGC182400.017.1603226
CGTATCA8224000.017.07384394-95
ACTGTCC146850.016.947938
CTAGACA264800.016.7351074
TACCGTG191350.016.5808727
TAGAGCG245000.016.3427945
GTATAAG406650.015.9778731
GACTGTG106400.015.7130467
CCGTATC9800150.015.46748994-95
Produced by FastQC (version 0.11.5)