FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_POK15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_POK15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95977405
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG98400.024.7138735
ATCATTA3211300.021.76574594-95
TCATTAA2532600.021.53345794-95
TATCATT3978600.020.02355694-95
TATACCG284900.019.5389445
CATTAAA1797950.019.36182694-95
GTCTAGG261450.018.5847511
GTATCAT4692900.018.48054194-95
GTACCGT150500.018.2413626
AGTACCG221350.017.3808675
ACTGTCC143200.016.7498158
GGACTGC192200.016.5077936
CCTAGAC275900.016.3717373
CTAGACA291600.016.28844
CGTATCA5578950.016.15811394-95
GTATAAG456200.016.0391431
GACGGTC127000.015.221327
TACACCG198750.015.1751345
GTGTATG347200.015.0237941
TACCGTG195700.014.9503567
Produced by FastQC (version 0.11.5)