FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_DHA60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_DHA60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100186621
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA4007750.024.9096994-95
CATTAAA2776200.023.74652594-95
ATCATTA5366400.023.54315894-95
TAGACGG95150.022.1136635
TATACCG247250.021.5921445
GTCTAGG236450.021.3355541
TATCATT6786700.021.0439794-95
GTACCGT151300.020.8132726
AGTACCG213000.019.288675
GTATCAT8338700.018.79760694-95
CTAGACA257400.018.6740174
ACTGTCC141300.018.5885498
TAGAGCG217900.018.1573945
CCTAGAC244250.017.6958963
TACACCG172700.017.5466465
TACCGTG189050.017.5113097
AAGACCG216400.017.1419225
GGACTGC189550.017.0893826
GTGTATG316900.016.9714551
GTATAAG377050.016.7656081
Produced by FastQC (version 0.11.5)