FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_DHA5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_DHA5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55281265
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2424150.016.1588994-95
CCGTATC1871400.015.75715494-95
CGCCGTA2966850.015.59040194-95
CGTATCA1544650.014.40042894-95
CATTAAA976000.013.29471794-95
TCATTAA1092500.012.71186894-95
TATACCG166900.011.9811985
TCGCCGT3734000.011.94781194-95
GTATCAT1391200.011.56861994-95
TGGTCGC4216000.011.1074990-91
ATCATTA1175300.010.85374492-93
GGTCGCC4240000.010.70509992-93
TATACTG241450.010.6228495
TAGAGCG150300.010.4602845
GTCGCCG4576400.010.376451592-93
TATCATT1308500.010.2770792-93
GTCTAGG151750.010.2602551
GTGGTCG4456550.010.13968490-91
CTAGACT161400.010.0940414
TAGACGG86600.010.0370865
Produced by FastQC (version 0.11.5)