FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_DHA5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_DHA5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44081537
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444120.10074966306188461No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT2199200.016.11325394-95
CGCCGTA2708600.015.74258994-95
CCGTATC1731800.015.477900594-95
CATTAAA893250.014.71813794-95
CGTATCA1417900.013.68793194-95
TCATTAA992950.013.49866894-95
TATACCG132950.012.7898135
TCGCCGT3405650.012.40610294-95
ATCATTA1084700.011.85671892-93
TGGTCGC3921900.011.10426990-91
GTCTAGA149100.011.0864781
GGTCGCC3926700.010.9810692-93
GTCTAGG121400.010.9478121
TATCATT1187700.010.8904792-93
TATACTG197400.010.731425
GTCGCCG4145000.010.65370592-93
GTATCAT1274850.010.57340894-95
GTGGTCG4128000.010.39106390-91
TAGAGCG111950.010.2249795
ATTAAAA1053900.010.218575594-95
Produced by FastQC (version 0.11.5)