FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_DHA180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_DHA180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153715933
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG162300.022.8559175
TATACCG445750.020.693035
GTCTAGG392500.020.5110511
ATCATTA3812450.020.00580294-95
TATCATT4749550.019.39874394-95
GTATCAT5700150.019.38139394-95
TCATTAA3008600.019.08807894-95
TAGAGCG395500.018.5304365
GTACCGT264250.018.36976
CGTATCA6955750.018.33530294-95
CCTAGAC402150.017.716153
TACACCG321300.017.6801785
AGTACCG366300.017.6347055
CCGTATC8498550.017.539294-95
ACTGTCC253950.017.2443038
GTCTATG487350.017.0567111
GGACTGC332350.016.9637246
CTAGACA478450.016.6877334
CATTAAA2215500.016.5821494-95
GTATAAG651950.016.5571861
Produced by FastQC (version 0.11.5)