FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_DHA180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_DHA180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87779754
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3612550.024.74665694-95
ATCATTA4858550.023.79595694-95
CATTAAA2525450.023.36706294-95
TATCATT6209450.021.51122594-95
TAGACGG85700.021.4485135
TATACCG206250.019.4363885
GTATCAT7649900.019.1420494-95
GTCTAGG198850.018.4689621
GTACCGT134200.018.3688476
ACTGTCC124600.017.6111838
CCTAGAC205450.016.784083
CGTATCA9278500.016.7185794-95
AGTACCG181900.016.6853475
GGACTGC166500.016.3458486
CTAGACA214500.015.8766534
GTGTATG266900.015.8302851
TACACCG156900.015.5901895
TACCGTG170650.015.5585847
TAGAGCG195100.015.532115
GTATAAG323100.015.2586831
Produced by FastQC (version 0.11.5)