FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_DHA15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_DHA15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120648874
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA6911400.026.1690694-95
CATTAAA4764650.025.56206194-95
ATCATTA9350600.024.42356194-95
TATACCG238550.021.9813635
GTACCGT140950.021.9034866
TATCATT11864400.021.80813294-95
TAGACGG98200.021.1365915
GTCTAGG255350.019.682651
GTATCAT14782050.019.08467994-95
ACTGTCC138750.018.998558
CTAGACA244900.018.6961964
AGTACCG206750.018.6771245
TAGAGCG234800.018.2866975
TACACCG174050.018.2837725
GGACTGC185150.018.2394146
TACCGTG176700.018.1975367
CCTAGAC259800.018.099273
ATACCGT266900.017.7601626
ATTAAAA3673850.017.34973794-95
ACTGAGC285700.016.940438
Produced by FastQC (version 0.11.5)