FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_BG760-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_BG760-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81746168
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG838570.1025821785309863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG98200.023.7001365
TATACCG256400.019.321135
GTACCGT168250.018.9141146
GTCTAGG245900.018.7137221
TCATTAA1560850.018.57162994-95
ATCATTA1926400.018.29769394-95
AGTACCG230450.017.993015
TAGAGCG261900.017.2650455
TATCATT2288800.017.13254494-95
CATTAAA1188500.017.00945394-95
CCTAGAC234850.016.7458043
GTATCAT2575000.016.67285394-95
GTCTATG292350.016.6202931
ACTGTCC171000.016.0266028
GTGTATG319150.015.6425681
GACGGTC131850.015.5259747
CGTATCA3000200.015.34941194-95
TACCGTG200300.015.2235457
CCGTATC3484800.014.93731494-95
TATACTG357800.014.934085
Produced by FastQC (version 0.11.5)