FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_BG760-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_BG760-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117014808
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1910630.16328104388292464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA3105650.023.1012294-95
ATCATTA4074950.022.74376994-95
TAGACGG125150.021.2911135
CATTAAA2167300.021.00272894-95
TATACCG309650.020.922315
TATCATT5103100.020.54828894-95
GTACCGT197650.019.7053176
GTCTAGG304650.019.171091
AGTACCG272550.018.6293565
GTATCAT6103200.018.54211294-95
CTAGACA338750.017.7509174
GTCTATG367500.017.5127961
CCTAGAC319400.017.2351443
TACCGTG242500.016.8441187
GTATAAG493550.016.7079581
CGTATCA7323800.016.35896394-95
ACTGTCC207150.016.3251298
TAGAGCG298750.016.057575
GGACTGC248500.016.0362196
GTGTATG405800.015.8716521
Produced by FastQC (version 0.11.5)