FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_BG75D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_BG75D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71805349
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2605770.3628935777472511No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC1320750.015.92310994-95
GCCGTAT1828100.014.68987194-95
CGTATCA1119100.014.65772794-95
CGCCGTA2278000.013.89697494-95
TATACCG208300.012.9288725
TAGAGCG257850.012.5811525
GTATCAT1011000.012.49413394-95
GTCTAGG221150.012.3863251
CCTAGAC209750.011.9789983
TCATTAA784600.011.54928794-95
GTCTTAG183300.011.513381
TAGACGG118550.010.7774885
CATTAAA725450.010.5166494-95
TGGTCGC3041550.010.45832790-91
GTATTGG433450.010.4191161
GTATAGG253800.010.3987171
TCGCCGT2961100.010.35576394-95
ATCATTA883150.010.27933994-95
TAGAGGT269950.010.2401534
CTAGACT180550.010.2070694
Produced by FastQC (version 0.11.5)