FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_BG7180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_BG7180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134184008
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2248320.16755498911613967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTA2751750.019.67686794-95
TATACCG339550.019.4715845
TCATTAA2136900.019.2083994-95
TATCATT3412300.019.18813994-95
GTATCAT4092350.019.08440294-95
GTCTAGG357050.018.7464871
TAGACGG168300.018.7391435
CGTATCA5010950.018.24838394-95
GTACCGT274200.018.240086
TAGAGCG373500.017.8415625
CCGTATC6169800.017.59693194-95
AGTACCG359900.017.36765
GTCTATG417750.016.8208581
CATTAAA1623350.016.63475494-95
GCCGTAT8045900.016.20439394-95
GTATAAG483300.016.1560341
ACTGTCC274800.016.0914388
CCTAGAC364550.015.6477433
CACTGAG602850.015.6076367
CGCCGTA9897400.015.60314394-95
Produced by FastQC (version 0.11.5)