FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_BG7180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_BG7180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137702473
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2265050.16448869440420288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA4541600.025.30863894-95
ATCATTA6210750.023.88893594-95
CATTAAA3099600.023.65872494-95
TATACCG343050.022.0282255
TATCATT7901600.021.51935894-95
GTACCGT230200.020.6948536
TAGACGG147000.020.582065
GTATCAT9769000.019.24855694-95
AGTACCG322150.019.0194665
GTCTAGG333500.018.911451
TAGAGCG355700.018.4006045
GTCTATG382200.017.5362241
ACTGTCC246600.017.3923138
TACCGTG287200.017.2489437
CTAGACA364900.016.9734634
GTATAAG483000.016.8450931
CGTATCA11833800.016.7645294-95
CCTAGAC356750.016.2828043
TACACCG283500.016.200945
GGACCGT335650.015.5375476
Produced by FastQC (version 0.11.5)