FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_BG715-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_BG715-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103031161
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1655820.160710602882559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATTAA2645100.022.8974894-95
TAGACGG119800.022.8366975
ATCATTA3453600.021.99376194-95
CATTAAA1871000.021.35172894-95
TATACCG271750.020.9913855
GTCTAGG276550.020.9630031
GTACCGT181850.020.2160026
TATCATT4277350.019.83439694-95
TAGAGCG266250.018.9631845
AGTACCG255550.018.5676635
GTATCAT5115550.018.19192994-95
CTAGACA292150.017.8998364
GTCTATG326300.017.3580671
ACTGTCC179150.017.2594838
CCTAGAC282400.017.0713923
GTGTATG366500.017.0658261
TACCGTG224450.016.8867827
GGACTGC232500.016.8742586
GTATAAG420300.016.7854211
TACACCG223000.016.7837495
Produced by FastQC (version 0.11.5)