FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n02_BG715-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n02_BG715-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124372100
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2322070.1867034487638305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG141650.022.6335415
ATCATTA3295450.021.4298994-95
TCATTAA2546850.021.3962994-95
TATCATT4089100.019.94841694-95
GTACCGT233050.019.871056
TATACCG343050.019.8544125
GTCTAGG342000.019.5156481
GTATCAT4820700.018.69331694-95
CATTAAA1851250.018.5889194-95
AGTACCG329100.017.8095345
GTCTATG402050.016.920731
CGTATCA5748850.016.40411894-95
CCTAGAC335900.016.1056793
TAGAGCG365700.016.0920585
CTAGACA379800.016.0448994
TACCGTG284400.015.9657977
GTATAAG521450.015.5130061
GTGTATG438200.015.4813421
GGACTGC275400.015.4183856
ACTGTCC233250.015.3740258
Produced by FastQC (version 0.11.5)