FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78832256
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG48700.017.4097865
GTCTATG112000.016.5655841
GTACCGT61350.015.9043846
AGTACCG84100.015.8826345
ACTGTCC63700.015.7633788
TACCGTG75250.014.7287017
CACTGAG139000.014.3457217
GTCTAGA107000.014.2797341
GTGTATG117300.013.9967161
GTATAGG127250.013.7972321
TAAGGTG110200.013.5823495
ACTGTGC82200.013.5409428
TCTTCTG2497650.013.05043594-95
GCATAGG88600.013.0142931
TAGAGGT102150.012.9839784
GTGCTAG85900.012.4842751
GTATAGA141800.012.4818981
AAGACCG96150.012.4142545
TACACCG85100.012.4120855
TGTACAG91650.012.351935
Produced by FastQC (version 0.11.5)