FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_POK60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_POK60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91545817
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2652300.025.03777594-95
CTTCTGC3702350.024.37098594-95
TAGACGG84600.023.8627835
GTACCGT132600.021.9949596
TCTGCTT1859500.021.94872794-95
TCTTCTG5090300.021.5001294-95
GTCTAGG220250.021.0968341
GTCTTCT6219900.019.51199794-95
GGACTGC169200.019.1742616
TATACCG227600.019.1381385
TACCGTG175900.018.7136147
CCTAGAC244450.018.5190323
AGTACCG199150.018.4851935
ACTGTCC122700.018.4655978
CTGCTTG1146200.018.39860394-95
GTATAAG383400.018.0303961
CTAGACA243800.017.185034
CGTCTTC7588800.017.1015294-95
TAGACCG117600.017.045425
AAGACCG206250.016.7894385
Produced by FastQC (version 0.11.5)