FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_POK5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_POK5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49177869
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2754700.016.51632194-95
TGCTTGA620900.015.86718794-95
TCTGCTT1013200.015.81840694-95
TGCCGTC3370850.015.78729594-95
GCTTGAA487250.015.73491994-95
CTGCTTG787650.015.54756994-95
CCGTCTT2190000.015.22236394-95
GTCTAGG134650.014.25503351
TTCTGCT1143450.014.24502494-95
TATACCG151650.013.9699165
ATGCCGT3766850.013.54563894-95
CCTAGAC133150.012.985973
GTATAGG231850.012.9714891
TAGAGCG137000.012.79402355
TATGCCG4166500.012.74561392-93
CTTCTGC1271750.012.67653192-93
CGTCTTC1929050.012.42311694-95
TATACTG220800.012.1333695
TAGACGG72450.012.0637065
ACTGTCC121450.011.7721988
Produced by FastQC (version 0.11.5)