FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_POK180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_POK180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98697120
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3000400.024.22682494-95
CTTCTGC4169900.023.44634694-95
TCTGCTT2194450.021.46249894-95
TCTTCTG5661200.020.99720494-95
GTACCGT151650.020.6727986
TAGACGG94050.020.5054235
GTCTAGG238750.019.3824961
TATACCG255750.019.1860755
GTCTTCT6910800.019.11001294-95
CCTAGAC252950.018.798013
ACTGTCC142400.018.446088
AGTACCG212550.018.2583895
CTGCTTG1378550.017.91818894-95
GTATAAG394400.017.0577011
CGTCTTC8496300.016.82084794-95
GGACTGC189250.016.7412416
TACCGTG196400.016.5668287
GTCTATG281000.016.4005931
AAGACCG221600.016.2051685
TAGAGCG243600.016.1651125
Produced by FastQC (version 0.11.5)