FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_POK15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_POK15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95977405
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG101950.026.0450745
TTCTGCT2292700.023.85352394-95
CTTCTGC3076850.022.97865794-95
TCTGCTT1678450.020.27771894-95
TCTTCTG4139150.019.93310594-95
GTCTAGG257800.019.3326571
TATACCG288150.018.495895
GTCTTCT4929100.018.09402594-95
GTACCGT157550.017.6675326
CCTAGAC279650.017.173163
ACTGTCC149400.017.009498
AGTACCG226900.016.9989665
GGACTGC211900.016.901966
GTATAAG458700.016.4793641
GACGGTC133800.016.4721817
CTAGACA296600.016.4159724
CTGCTTG1073600.016.23808794-95
GTGTATG351450.016.1413481
ACTGAGC315250.015.8808458
CGTCTTC5849550.015.82910594-95
Produced by FastQC (version 0.11.5)