FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_DHA60-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_DHA60-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100186621
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3959600.025.61354494-95
TAGACGG93950.024.5216435
CTTCTGC5438800.023.7493194-95
TCTGCTT2786850.023.74466594-95
GTCTAGG232550.022.5557841
GTACCGT149850.021.3015546
TCTTCTG7241100.020.6179794-95
ACTGTCC146750.020.2945048
CTGCTTG1729850.020.20525494-95
TATACCG271450.020.0364275
AGTACCG204150.019.2657345
CCTAGAC252600.018.4671763
TACCGTG195950.018.4471767
CTAGACA270950.018.4262074
GTCTTCT8807600.018.41060394-95
GTATAAG374100.018.3266811
TAGAGCG223250.018.1493955
TACACCG178500.017.936035
GGACTGC190800.017.7753686
GTGTATG317300.017.354761
Produced by FastQC (version 0.11.5)