FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_DHA60-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_DHA60-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154231150
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT6359500.024.80717394-95
TCTGCTT4540900.023.19970194-95
CTTCTGC8669850.023.15885294-95
TCTTCTG11532150.020.42657594-95
CTGCTTG2891700.019.5358294-95
TAGACGG156050.018.9029715
TATACCG386300.018.5921385
GTCTTCT13970200.018.23427894-95
GTCTAGG344350.018.116131
CCTAGAC374150.016.8857783
GTACCGT244750.016.7877316
GGACTGC306600.016.7630756
ACTGTCC243500.016.3273378
GTATAAG572550.016.0571461
CGTCTTC16753750.015.76400994-95
AGTACCG328600.015.7565535
TAGAGCG354750.015.6796645
TACACCG305200.015.4393645
TGCTTGA2057900.015.22653494-95
TACCGTG319100.015.1236747
Produced by FastQC (version 0.11.5)