FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_DHA5D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_DHA5D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55281265
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT621000.013.48539494-95
GCCGTCT630350.013.070694-95
TGCCGTC988700.012.85655294-95
TTCCGTC586800.012.51475494-95
TATACCG170150.011.8088395
TCCGTCT437950.011.46445594-95
ATGCCGT796850.011.43636894-95
GTCTAGG158300.011.37420751
TATGCCG917900.011.22420492-93
TAGACGG88050.011.2210315
ATTCCGT542150.010.8906794-95
ACTGAGC249350.010.8200568
TATTCCG603500.010.578238592-93
CGTATGC1120250.010.52817690-91
CCTAGAC152950.010.4351433
TATACTG242100.010.2809995
GTATGCC1088250.010.10447392-93
ACTGTCC144800.010.103528
TAGACTG155400.09.9952945
CGTATTC790350.09.81727790-91
Produced by FastQC (version 0.11.5)