FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_DHA5D-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_DHA5D-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44081537
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA457100.016.32067394-95
GCCGTCT2313200.015.81310494-95
TGCTTGA587950.015.64142694-95
TGCCGTC2835850.015.29573194-95
CCGTCTT1854850.014.70758694-95
CTGCTTG714600.014.657494-95
TCTGCTT896200.014.37991194-95
ATGCCGT3155050.013.43658994-95
TTCTGCT1019000.013.01991594-95
TATGCCG3477850.012.45996492-93
GTCTAGG111850.012.106361
CGTCTTC1666850.011.98057394-95
TATACCG135750.011.897125
CTTCTGC1110750.011.71507292-93
TATACTG195100.011.613525
TAGAGCG119900.011.4493665
GTATGCC3734150.011.38532692-93
CGTATGC3836950.011.28331590-91
CCTAGAC120700.011.1770993
GTCCTGG161000.010.9189431
Produced by FastQC (version 0.11.5)