FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_DHA180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_DHA180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153715933
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG169200.025.8559745
GTCTAGG393000.022.4620761
TATACCG456950.021.5596495
GTACCGT266600.020.5253056
CTTCTGC2864500.020.08611794-95
TAGAGCG390650.020.0630875
AGTACCG367000.019.1168375
GTCTTCT4514550.018.76959494-95
ACTGTCC251950.018.7584978
GGACTGC334750.018.7021716
GTCTATG493850.018.6639421
TCTTCTG3796000.018.57592294-95
TTCTGCT2069200.018.52257594-95
TACACCG320500.018.4667995
CCTAGAC415250.018.4175133
TACCGTG348150.017.9820677
CGTCTTC5997400.017.86647894-95
GTGTATG583800.017.7088991
GTATAAG670050.017.7054941
ACTGAGC539650.017.5070088
Produced by FastQC (version 0.11.5)