FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_DHA180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_DHA180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87779754
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT3220100.025.35501594-95
CTTCTGC4532600.023.98605794-95
TCTGCTT2275600.023.44914694-95
TAGACGG87550.021.9193385
TCTTCTG6055700.021.04809494-95
GTCTAGG194800.020.4873961
TATACCG213550.019.7522055
CTGCTTG1424300.019.73867894-95
GTACCGT133400.019.6910026
GTCTTCT7400300.018.76909694-95
AGTACCG179000.018.310425
ACTGTCC123650.017.747518
CCTAGAC216600.017.1499313
TACCGTG172850.016.9553247
GGACTGC171300.016.9149596
GTCTATG238700.016.6597881
CGTCTTC9222050.016.47144594-95
GTATAAG329500.016.4234661
GTCTAGA201750.015.9430561
TACACCG164950.015.780855
Produced by FastQC (version 0.11.5)