FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_DHA15-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_DHA15-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120648874
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT7063250.026.7477594-95
TCTGCTT4845850.025.96482394-95
CTTCTGC9779800.024.4753494-95
TAGACGG90900.023.5675415
GTACCGT139450.023.4691936
TATACCG242700.023.2317815
CTGCTTG2926000.022.67397394-95
TCTTCTG13129000.021.21374994-95
TACCGTG178600.021.1700067
ACTGTCC139350.020.3837018
CTAGACA244500.020.3803714
AGTACCG207950.019.9414585
GTCTAGG239400.019.866161
GGACTGC183750.019.2586756
GTCTTCT16062600.018.72886394-95
CCTAGAC281250.018.6631623
ACCGTGA234600.018.6474938
TAGAGCG229700.018.5081775
GTCTATG266500.018.1847341
ATACCGT277250.018.1435346
Produced by FastQC (version 0.11.5)