FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_DHA15-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_DHA15-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111028808
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2819400.022.35268694-95
CTTCTGC3657800.021.4907994-95
TCTGCTT2102600.019.81667994-95
TAGACGG127850.019.6542175
TCTTCTG4820600.018.54325994-95
GTCTAGG280850.017.8644811
TATACCG316350.017.7480415
GTCTTCT5662700.017.16603794-95
CTGCTTG1371250.016.6331394-95
CCTAGAC298850.015.9427533
GTACCGT200800.015.8492456
AGTACCG258650.015.7203455
CGTCTTC6767250.015.51257594-95
ACTGTCC192450.015.2778338
GTATAAG486100.015.2084481
CCGTCTT7826750.014.60889494-95
GGACTGC249750.014.3785056
CTAGACA355350.014.2099524
GTGTATG414850.014.2014121
ACTGAGC366200.014.1383018
Produced by FastQC (version 0.11.5)