FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_BG760-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_BG760-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81746168
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG95650.022.148765
GTCTAGG240850.021.7189181
TTCTGCT1428300.020.35038494-95
TATACCG270000.019.9502725
GTACCGT176000.019.1080976
CTTCTGC1893650.018.96792694-95
AGTACCG241650.018.457735
CCTAGAC248800.018.2143173
GTCTATG295700.017.6580981
TCTTCTG2342400.017.43401794-95
TCTGCTT1160350.017.21631494-95
TAGAGCG279800.017.1124555
ACTGTCC169300.016.5532348
CACTGAG447950.016.5206787
ACTGAGC408650.016.5053468
GGACTGC188950.016.214736
GTCTTCT2726200.016.19234794-95
GTATAAG386600.016.1730381
TATACTG370000.015.9961635
GTGTATG320350.015.8544261
Produced by FastQC (version 0.11.5)