FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_BG760-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_BG760-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117014808
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG130500.025.5520365
TTCTGCT2915700.024.35070694-95
CTTCTGC4051250.022.9764194-95
GTCTAGG318600.022.2046811
TATACCG314400.022.1488085
TCTGCTT2119300.020.97180794-95
GTACCGT200450.020.6873156
TCTTCTG5273450.020.43650894-95
AGTACCG280850.019.1964245
CCTAGAC322100.018.8032233
ACTGTCC202550.018.4557138
GTCTTCT6347550.018.19779494-95
GGACTGC252300.018.073826
TACCGTG250000.018.0688117
GTATAAG485400.017.902331
GTCTATG357150.017.7859211
TACACCG254250.017.7298135
TAGAGCG302400.017.4357285
GTGTATG406850.017.306511
CTAGACA350350.016.866744
Produced by FastQC (version 0.11.5)