FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_BG75D-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_BG75D-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71805349
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1832650.015.08017394-95
CCGTCTT1469450.014.598694-95
TGCCGTC2261650.014.08163694-95
GTCTAGG213300.013.1421271
TATACCG214050.012.8710465
TAGAGCG259700.012.8583275
ATGCCGT2395450.012.79438394-95
TAGACGG117250.012.5588515
TATGCCG2621250.012.08397792-93
CGTCTTC1421600.012.01751894-95
CCTAGAC220300.011.6869043
GTCTTCT1148250.011.4985394-95
TATACTG276200.011.2474785
GTCTATG266100.011.1415141
GTACCGT208700.011.0841446
GTATAGG255050.011.083991
TTCTGCT824250.010.97574894-95
CTAGACA303900.010.9728924
GTGTATG320150.010.907831
GTATGCC2858900.010.7505192-93
Produced by FastQC (version 0.11.5)