FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_BG7180-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_BG7180-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134184008
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG363250.020.717921
TATACCG349050.020.399245
TAGAGCG364650.019.4614125
TTCTGCT2184300.019.33090494-95
TAGACGG171550.019.0778085
CTTCTGC2964400.018.99421594-95
GTCTATG416950.018.7560961
TCTTCTG3723900.018.7244994-95
GTCTTCT4499350.018.29828894-95
GTACCGT276400.017.7181026
AGTACCG344650.017.6551275
CCTAGAC365400.017.2510223
CCGTCTT6681150.017.07216894-95
ACTGTCC275050.017.062178
CGTCTTC5678700.016.92812294-95
ACTGAGC577850.016.7853188
CACTGAG625100.016.717177
GCCGTCT8278750.016.56909894-95
TACACCG324650.016.3578555
GTATAAG465700.016.0479321
Produced by FastQC (version 0.11.5)