FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_BG7180-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_BG7180-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137702473
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT4521950.025.48167694-95
CTTCTGC6378950.023.5945894-95
TATACCG340300.022.8919665
TCTGCTT3157950.022.76966394-95
TAGACGG148100.021.2967915
TCTTCTG8360300.021.10873494-95
GTCTAGG344700.020.467951
GTACCGT244250.019.9725386
TAGAGCG350600.019.3065265
AGTACCG325000.018.8541745
GTCTTCT10238500.018.8305894-95
ACTGTCC245500.018.6902458
GTCTATG396050.018.0420951
CTGCTTG2053850.017.91845794-95
GTATAAG484150.017.6146371
TACACCG289200.017.2133035
TACCGTG290750.017.1047767
ACTGAGC506700.016.6206178
CCTAGAC357350.016.5496483
CACTGAG540500.016.5391967
Produced by FastQC (version 0.11.5)