FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_BG715-W-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_BG715-W-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103031161
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG125550.024.4785375
TATACCG294500.023.0808735
GTCTAGG280650.022.532531
GTACCGT191850.021.9364646
AGTACCG263100.020.5456225
GTCTATG315100.019.3603671
TACCGTG235100.018.8300787
TACACCG221700.018.5973875
CTAGACA305250.018.596294
CACTGAG429750.018.557687
CCTAGAC289700.018.4466783
GTATAAG424100.018.3726831
ACTGAGC393800.018.285738
TCTGCGT307850.018.19989294-95
CTTCTGC1552600.018.12753794-95
TAGAGCG265350.018.1158755
GTGTATG376600.017.9271281
TATACTG405900.017.5186525
ACTGTCC183600.017.41138
GTCTAGA265550.016.9612961
Produced by FastQC (version 0.11.5)