FastQCFastQC Report
Fri 2 Oct 2020
HKKLVDRXX_n01_BG715-S-R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKLVDRXX_n01_BG715-S-R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124372100
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT2448850.022.95131594-95
CTTCTGC3387000.021.67882394-95
TATACCG352650.021.0127245
GTCTAGG331400.020.8599911
GTACCGT241900.020.009476
TCTTCTG4361950.019.87874694-95
TCTGCTT1867450.018.80797894-95
AGTACCG331300.018.6247045
GTCTTCT5213800.018.21525494-95
CCTAGAC348700.018.1045823
TAGACGG183500.017.8354825
GTCTATG395650.017.5926041
TAGAGCG363350.017.3217755
CTAGACA379100.017.2792914
ACTGTCC243000.016.6345948
TACACCG265550.016.5819325
ACTGAGC552450.016.559698
GGACTGC281700.016.5079526
TATACTG520900.016.4233235
GTATAAG529350.016.4072911
Produced by FastQC (version 0.11.5)