FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25403130
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6194072.438309767339694No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2361440.9295862360268202Illumina Single End PCR Primer 1 (100% over 51bp)
ATAACTGAAGATCATACTTTAGCTAAACAGATAAAATAACGGAGGTCGCCG1523000.5995324198238563No Hit
ATAACTGAAGATCATACTTTAGCTAAACAGATAAAATAACGGTGGTCGCCG1320270.5197272934476972No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAGATCGGAAGAGCGTCGTGTA674780.26562868433929204Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATAACTGAAGATCATACTTTAGCTAAACAGATAATATAACGGTGGTCGCCG674360.26546335038241353No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCAGATCGGAAGAGCGTCGTGTAG667600.2628022609812255Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG615450.24227329466880657No Hit
ATAACTGAAGATCATACTTTAGCTAAACAGATAATATAACGGAGGTCGCCG593140.2334909123403297No Hit
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGAA486520.19151970642987695Illumina Single End PCR Primer 1 (100% over 26bp)
ATAACTGAAGATCATACTTTAGCTAAACAGATAAGATAACGGTGGTCGCCG459920.18104855582756926No Hit
ATAACTGAAGATCATACTTTAGCTAAACAGATAAGATAACGGAGGTCGCCG408770.16091324179343255No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAAGATCGGAAGAGCGTCGTGT390110.1535676902806859Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATAACTGAAGATCATACTTTAGCTAAACAGATAAAAAAACGGAGGTCGCCG371790.1463559805425552No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCGACTAGATCGGAAGAGCGTCGT370180.14572220037452077Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG356200.14021894152413503No Hit
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAAA326430.12849991319967266Illumina Single End PCR Primer 1 (100% over 27bp)
ATAACTGAAGATCATACTTTAGCTAAACAGATAAAATCACGGTGGTCGCCG326240.12842511926679903No Hit
ATAACTGAAGATCATACTTTAGCTAAACAGATAAAATCACGGAGGTCGCCG271690.10695138748650265No Hit
GTGACTGGAGTTCAGACGTGTGCTCTAGATCGGAAGAGCGTCGTGTAGGGA261240.1028377211784532Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTA1824600.044.62183819
GCTAAAC1883650.044.5682422
TAGCTAA1993350.044.4439420
TAACTGA2236850.044.35532
ACGGAGG690050.044.33658639
TCATACT1917550.044.3300612
CATACTT1773900.044.26838313
ACTTTAG1815950.044.24817316
TTCGCCG223550.044.2348745
CGGAGGT758350.044.20335440
GATCATA1949450.044.16261710
AGATCAT2171400.044.159699
ACTGAAG2185100.044.1376574
GAGGTCG756300.044.0653942
TAAACAG1890600.044.0509524
GGAGGTC766200.043.9501541
ACGGTGG753600.043.9087439
ATCATAC1961250.043.9044211
TAACGGT556850.043.8600837
AACGGAG730450.043.85281438
Produced by FastQC (version 0.11.5)