FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds2124-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds2124-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18813450
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5580502.9662289479069495Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG783550.4164839516409803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG618650.043.04674545
GGTCGCC650500.040.9356244
GTGGTCG665000.039.90082642
TGGTCGC680400.039.00774843
GGTGGTC685150.038.74972541
CGGTGGT696900.038.13836340
CTCGGTG703950.037.87786538
TCTCGGT711950.037.60323337
TCGGTGG719700.037.12064739
ATCTCGG723500.037.03092236
GATCTCG764700.035.0299135
AGATCTC805950.033.55805234
GCACACG66250.032.7051518
TAGATCT848100.031.91693733
CACACGT58400.030.97595819
AGTGTAG860350.030.87433229
GAGTGTA866550.030.70260628
GTAGATC877200.030.34539832
GTGTAGA880600.030.19757330
TGTAGAT890750.029.90399631