FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds2123-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds2123-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17590204
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7320094.161458275299138Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG728970.414418161381187No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG798050.043.2686345
GGTCGCC835100.041.31957644
GTGGTCG856750.040.21479842
TGGTCGC872100.039.4917243
GGTGGTC878300.039.21216641
CGGTGGT888500.038.80758740
CTCGGTG891900.038.7175638
TCTCGGT899950.038.4231937
TCGGTGG905650.038.17445439
ATCTCGG913650.037.89875436
GATCTCG952650.036.34970535
AGATCTC992600.035.0929834
TAGATCT1031750.033.77881233
AGTGTAG1047100.032.88191629
GAGTGTA1054200.032.73079328
GTAGATC1058350.032.5919132
GTGTAGA1064050.032.42366430
TGTAGAT1070250.032.2337331
AGAGTGT1090300.031.64922327
AAGAGTG1104950.031.29272526