FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds2113-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds2113-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12884274
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2086451.619377234603983Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG374790.29088949831399113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG236650.042.49975645
GGTCGCC244000.041.11826744
GTGGTCG255900.039.1091442
TGGTCGC258350.038.74726543
CTCGGTG261150.038.33925238
ATCTCGG267650.037.49135636
TCTCGGT268450.037.32919737
GGTGGTC270050.037.0258641
TCGGTGG275700.036.4383239
CGGTGGT277750.036.16951840
GATCTCG283600.035.32767535
AGATCTC301700.033.49163434
GCACACG39300.032.86166818
TAGATCT316700.031.89114633
AGTGTAG315400.031.60171129
GAGTGTA320550.031.17084328
GTAGATC324150.030.8598432
GTGTAGA328000.030.52505330
TGTAGGG334550.030.0215718
CGTGTAG337800.029.85928216