FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds2102-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds2102-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18307636
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2730521.4914650913968357Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG276270.1509042456382681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG307000.042.42706345
GGTCGCC319200.040.6928144
GTGGTCG340450.038.07335342
GCACACG93500.036.8894718
TGGTCGC355450.036.45419743
GGTGGTC359600.036.0076841
CTCGGTG363800.035.6910338
ATCTCGG370200.035.24350436
TCTCGGT372600.034.937937
CGGTGGT372450.034.8257840
TCGGTGG383800.033.89554239
GATCTCG387450.033.65715835
AGCACAC115200.033.4366817
CACACGT66300.032.4090619
GAGCACA147750.032.2377916
AGATCTC408550.032.20526534
AGAGCAC150250.032.00087715
TAGATCT423100.031.09784133
AGTGTAG427350.030.25724429
GAGTGTA438950.029.57529628