FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds2098-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds2098-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19421180
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG971170.500057154096713Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233420.12018837166433759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG123700.039.85213545
GGTCGCC126850.038.8446744
GTGGTCG143900.034.1013542
CTCGGTG145350.033.7916838
ATCTCGG148700.033.1811136
GCACACG62550.033.12896718
TGGTCGC149050.032.83258443
TCTCGGT151750.032.48455437
GATCTCG160450.030.69519835
TCGGTGG163150.030.42212139
GGTGGTC164750.029.7579441
CACACGT58400.028.66407219
CGGTGGT173250.028.4018540
AGCACAC83050.028.17534617
ACACGTC48150.027.47633720
AGATCTC183650.027.27084534
GAGCACA98400.026.7983516
AGAGCAC101200.026.23475815
AGTGTAG190650.025.72676529
TAGATCT200400.025.08128733
Produced by FastQC (version 0.11.5)