FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds2092-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds2092-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14609290
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5473613.746663937809435Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG478800.32773666618980113No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG611500.043.5319445
GGTCGCC638400.041.61997244
GTGGTCG660150.040.19063642
TGGTCGC671900.039.4644943
GGTGGTC675550.039.25738541
CTCGGTG679500.039.1914338
CGGTGGT678750.039.07561540
TCTCGGT687650.038.79144337
TCGGTGG690450.038.59921339
ATCTCGG695550.038.41567636
GATCTCG723550.036.91973535
AGATCTC753100.035.74295434
TAGATCT778050.034.64026633
GCACACG48400.034.26046818
AGTGTAG781800.033.9129229
GAGTGTA792300.033.55992528
GTAGATC797400.033.35397732
GTGTAGA802850.033.07431430
TGTAGAT809600.032.86525731
AGAGTGT818350.032.4971327