FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds2089-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds2089-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17722726
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2698631.5226946464104902Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316190.17840934853927098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG309100.042.5824545
GGTCGCC322250.040.82385344
GCACACG136250.039.1370518
GTGGTCG339350.038.54129442
AGCACAC159950.036.8123317
TGGTCGC355900.036.78066343
GGTGGTC360350.036.319741
GAGCACA201850.036.01488516
AGAGCAC205850.035.55561415
CGGTGGT370150.035.39457340
CACACGT90500.035.22897319
CTCGGTG378700.034.5420938
TCTCGGT384400.034.10540437
ATCTCGG386650.033.95959536
ACACGTC66900.033.56472820
TCGGTGG393100.033.35678539
GATCTCG402650.032.79491835
CACACGA63800.032.0569319
ACACGAG62700.032.04517420
AGATCTC425350.031.3885534
Produced by FastQC (version 0.11.5)