FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds2086-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds2086-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22540348
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG611810.27142881733680424Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232080.10296203057734513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG93950.037.95877545
GGTCGCC97400.037.1455544
GCACACG72150.035.3005218
CACACGT67600.031.85232419
GTGGTCG105300.031.55941242
ACACGTC60600.030.44507620
ATCTCGG101550.030.22029736
CTCGGTG100750.030.21487838
AGCACAC91550.029.73692917
TCTCGGT105200.028.97916837
AGAGCAC108150.027.54428915
GATCTCG113600.027.27236435
GAGCACA108200.027.21958216
CACGTCT49800.026.97229621
TGGTCGC127100.026.0403543
GGTGGTC129350.025.7260341
TCGGTGG124550.025.03730839
AGATCTC132500.023.85764734
CGGTGGT143300.023.41005540
CGGGGAC17150.021.795981
Produced by FastQC (version 0.11.5)