FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1793-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1793-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24017265
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4248841.7690773699669802Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG363240.15124120086113052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG482450.043.1062945
GGTCGCC496900.041.8075544
TGGTCGC516300.040.136443
GTGGTCG517050.040.07374242
CTCGGTG521750.039.81110838
TCTCGGT532700.039.03418737
ATCTCGG539700.038.55724736
GCACACG136850.038.4555518
GGTGGTC544250.038.15286341
TCGGTGG545700.038.1379939
CGGTGGT555450.037.38760840
GATCTCG569800.036.56410635
CACACGT121750.036.4800519
ACACGTC107400.035.09035520
AGCACAC167500.034.60213517
AGATCTC620200.033.7886534
GAGCACA194150.033.20160316
AGAGCAC195150.033.1929515
CACGTCT87850.032.55184621
TAGATCT662900.031.63255733
Produced by FastQC (version 0.11.5)