FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1793-05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1793-05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24999949
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8942933.5771792974457672Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG600580.24023249007427977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG987700.043.6512845
GGTCGCC1023500.042.0869944
TGGTCGC1047050.041.0458343
GTGGTCG1048000.041.00209842
CTCGGTG1062400.040.46687738
TCTCGGT1070750.040.2302737
GGTGGTC1072900.040.05632841
ATCTCGG1085100.039.75629436
TCGGTGG1083750.039.719539
CGGTGGT1087150.039.5374940
GATCTCG1128750.038.2053135
GCACACG125650.037.81854218
AGATCTC1198200.036.20303734
CACACGT102550.034.8191519
TAGATCT1262050.034.38755833
AGCACAC157250.033.78148317
AGTGTAG1314150.032.68558529
GAGCACA190300.032.4191816
GTGTAGA1330350.032.319730
GTAGATC1332800.032.3024932