FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1793-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1793-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22817593
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG11040424.838555933572835Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG583860.255881503364531No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1230850.043.8278645
GGTCGCC1273200.042.3293944
GTGGTCG1298000.041.4235642
TGGTCGC1298350.041.40719243
CTCGGTG1319200.040.80149538
GGTGGTC1325400.040.57498641
TCTCGGT1332650.040.4918937
TCGGTGG1343150.040.2162539
CGGTGGT1338200.040.19865440
ATCTCGG1350800.040.02794636
GCACACG141350.038.63223318
GATCTCG1402150.038.41296835
AGATCTC1481350.036.56730734
CACACGT109000.035.3597819
AGCACAC172500.035.12539317
TAGATCT1556350.034.8312433
GAGCACA213750.034.430916
AGAGCAC216600.034.11297615
ACACGTC89550.033.71829620
AGTGTAG1630900.032.88459429