FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1793-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1793-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24991169
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG262062410.48620014533934Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1272330.5091118386658903No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG358970.14363873894814605Illumina Single End PCR Primer 1 (98% over 51bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC253180.10130778596231334Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG2889600.044.04266445
GGTCGCC2978000.042.68684444
TGGTCGC3009150.042.1141143
GTGGTCG3009850.042.1072242
GGTGGTC3035000.041.75952541
CGGTGGT3047300.041.60721640
CTCGGTG3083200.041.2100738
TCTCGGT3099350.041.04742437
TCGGTGG3105700.040.96077739
ATCTCGG3142600.040.55283736
GCACACG242000.040.5083918
GATCTCG3254600.039.07933435
CACACGT172250.038.54661619
AGCACAC289000.038.20235417
GAGCACA361750.037.84634416
AGATCTC3386250.037.7812734
ACACGTC136950.037.26123420
AGAGCAC371200.037.0707515
TAGATCT3519650.036.35449233
CACACGA83350.034.98449319