Basic Statistics
Measure | Value |
---|---|
Filename | HKKJCDRXX_l02_n02_ds1793-03.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 24991169 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 2620624 | 10.48620014533934 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 127233 | 0.5091118386658903 | No Hit |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 35897 | 0.14363873894814605 | Illumina Single End PCR Primer 1 (98% over 51bp) |
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 25318 | 0.10130778596231334 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 288960 | 0.0 | 44.042664 | 45 |
GGTCGCC | 297800 | 0.0 | 42.686844 | 44 |
TGGTCGC | 300915 | 0.0 | 42.11411 | 43 |
GTGGTCG | 300985 | 0.0 | 42.10722 | 42 |
GGTGGTC | 303500 | 0.0 | 41.759525 | 41 |
CGGTGGT | 304730 | 0.0 | 41.607216 | 40 |
CTCGGTG | 308320 | 0.0 | 41.21007 | 38 |
TCTCGGT | 309935 | 0.0 | 41.047424 | 37 |
TCGGTGG | 310570 | 0.0 | 40.960777 | 39 |
ATCTCGG | 314260 | 0.0 | 40.552837 | 36 |
GCACACG | 24200 | 0.0 | 40.50839 | 18 |
GATCTCG | 325460 | 0.0 | 39.079334 | 35 |
CACACGT | 17225 | 0.0 | 38.546616 | 19 |
AGCACAC | 28900 | 0.0 | 38.202354 | 17 |
GAGCACA | 36175 | 0.0 | 37.846344 | 16 |
AGATCTC | 338625 | 0.0 | 37.78127 | 34 |
ACACGTC | 13695 | 0.0 | 37.261234 | 20 |
AGAGCAC | 37120 | 0.0 | 37.07075 | 15 |
TAGATCT | 351965 | 0.0 | 36.354492 | 33 |
CACACGA | 8335 | 0.0 | 34.984493 | 19 |