FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1793-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1793-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26871466
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10422023.8784709401414865Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG756780.2816295917759009No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1167750.043.61050445
GGTCGCC1204600.042.22411344
GTGGTCG1228050.041.33164242
TGGTCGC1231250.041.23343343
CTCGGTG1251050.040.72029538
TCTCGGT1262500.040.38038337
GGTGGTC1261650.040.23558441
ATCTCGG1268500.040.21436336
CGGTGGT1268350.040.0372340
TCGGTGG1277000.039.95624539
GATCTCG1310750.038.87184535
GCACACG127900.037.98024418
AGATCTC1367850.037.56497234
TAGATCT1423150.036.11017233
AGTGTAG1455900.034.8190829
GAGTGTA1470800.034.60395428
GTAGATC1474450.034.41618332
GTGTAGA1478050.034.35214630
CACACGT96600.034.28555319
TGTAGAT1496300.033.9151231